This web page was produced as an assignment for Gen677 at UW-Madison Spring 2012.
Human ITGAM protein domains
The structure of a protein is important for its function. Below is human ITGAM protein structural information.
Secondary structure
The secondary structure above was obtained from UniProt. It shows the secondary structures found in the human ITGAM protein. It is mostly comprised of helix and beta strand structures and contains one turn structure.
ITGAM protein domains
Protein domains are conserved functional parts of a protein sequence. They have the ability to function individually on their own without being part of the complete protein. ITGAM protein domains were analyzed in Pfam and SMART.
Pfam domain
SMART domain
Domain descriptions
vWA - Von Willebrand factor type A domain (yellow box in Pfam and blue pentagon in SMART)
is found in various plasma proteins
involvement in multiprotein complexes
number of human diseases arise from mutations in VWA domains
fold predicted to be a doubly wound, open, twisted beta-sheet flanked by alpha-helices
proteins that incorporate vWF domains participate in numerous biological events
domain adopts a classic alpha/beta Rossmann fold
contains an unusual metal ion coordination site at its surface
FG-GAP repeat (blue box in Pfam)
No annotation
member of clan Beta_propeller
Integrin alpha domain (red box in Pfam and pink pentagons in SMART)
receptor
mediate attachment between a cell and the surrounding tissues (other cells or extracellular matrix)
has a role in cell signaling -- regulating cellular shape, motility, & the cell cycle
Integrin alpha cytoplasmic region (green box in Pfam and blue box in SMART)
cytoplasmic integrin domain
receptor
mediate attachment between a cell and the surrounding tissues (other cells or extracellular matrix)
has a role in cell signaling -- regulating cellular shape, motility, & the cell cycle
Analysis
Pfam found one morel domain (FG-GAP) in human ITGAM than SMART. The additional domain is a domain that has not been annotated yet, meaning that the function of the domain is unknown. This is a likely reason why it was not found in both databases.
Domains found in Pfam and SMART support the gene ontology description for ITGAM. The integrin domains are the "receptor and cell signalling part" of the protein and give ITGAM its receptor and signaling activity. The vWA domain may allow for ITGAM's involvement in blood coagulation as described on the Gene ontology page. However, the domain may give ITGAM other functions as well because the domain has been found in proteins involved in numerous biological functions.